Abstract

ANTI-MICROBIAL PROFICIENCY OF PHYTOCHEMICAL EXTRACTION IN METHANOL FROM SOME MEDICINAL PLANTS

Asha D. Lazras, *Anil Kumar, B.D. Nagle and Akansha Gayakwad

Abstract

The some medicinal plants part has antimicrobial properties such as Azadirachta indica (Neem), Ocimum scantum (Tulsi), Curcuma longa (Turmeric), Annona squamosal (Sugar Apple), Allium sativum (Garlic) etc. its leaves and stem used for phytochemicals extraction in methanol. Each sample has divided into two replicates for extraction in hot and cold methanol. Extracted phytochemicals applied to TLC plate for separation of various compounds and know their Rf values of each separated bands on TLC plate, pigments parts dissolved in distilled water, and its mix with culture media of plates, carry on open to plates in air environment for 5min. and incubated at 37ºC for 24-48 hours, observed antimicrobial effect to compared with control plate. In which maximum 194 colonies inhibited by dark green phytochemical of leave Azadirachta indica and another maximum 188 colonies inhibited by yellow pigment which Rf value 0.1825 of stem bark of Azadirachta indica and light green which Rf value 0.2625 of stem Ocimum scantum and less effective 8 colonies inhibited by unknown pigment of stem bark of Azadirachta indica from out of 300 colonies of control plate.

Keywords: Antimicrobial, Methanol, Rf. Value, Soxhlet and TLC.