Abstract

EVALUATION OF ANTIBACTERIAL ACTIVITY OF PHOSPHATE ESTERS BY WELL DIFFUSION METHOD

Mithilesh Kumari Gupta, S. K. Jadhav, S. A. Bhoite*

Abstract

Resistance in some pathogenic bacterial strains to conventionalantibiotics has initiated to search for effective treatments againstmicroorganisms. In this context antibacterial activity of mono-6-chloro-2,4-dinitroaniline phosphate and di-2-methyl-5-nitroanilinephosphate were studied by determining the zone of inhibition ofbacterial growth against four pathogenic bacterial strainsStaphylococcus aureus MTCC 3160, Klebsiella oxytoca ATCC 13182,Bacillus subtilis BAB 2437 and Bacillus licheniformis MS 17.Different concentrations of test compounds were applied againstbacterial strains to examine their antibacterial activity by using welldiffusion method. Gentamycin and water were taken as a positive andnegative control to test the bioactivity of phosphate esters. Zone of inhibition was observedand measured in mm. Minimum inhibitory concentration (MIC) of phosphate esters againstpathogenic bacterial strains was also determined.

Keywords: Mono-6-chloro-2,4-dinitroaniline phosphate, di-2-methyl-5-nitroaniline phosphate, antibacterial activity, well diffusion method, zone of inhibition, MIC.