Abstract

RP-HPLC FORCED DEGRADATION STUDIES OF NIMODIPINE IN PHARMACEUTICAL DOSAGE FORM

Sonali G. Lahamage*, Dhamak Vikrant M. and Dhamak Kiran B.

Abstract

Introduction: Nimodipine is a calcium channel blocker used for the treatment of hypertension, angina and cardiac arrhythmia. The research work proposes the development of LC methods for the estimation of Nimodipine in pharmaceutical dosage form and forced degradation studies on four different stress conditions. Method: The LC20AD Prominence Liquid Chromatograph SPD20-A Shimadzu, Japan with UV-Vis detector and C18 column with dimension on 25 x 0.6 cm was used for the method development with flow rate 1.0 ml/min at wavelength 236 nm. The methanol: acetonitrile: water in proportion of (35v:38v:27v) as a mobile phase, for development of chromatogram. Conclusion: Degradation studies disclose method abilities on various stress conditions. Forced degradation results can be used for the development of stable dosage form and for the designing of proper storage requirement. The proposed method is accurate, precise, specific and rapid for the estimation of Nimodipine.

Keywords: Nimodipine, RP- HPLC, Forced degradation studies, Method development.