Abstract

PHYTOCHEMICAL SCREENING AND IN VITRO ANTI ELASTASE, ANTI COLLAGENASE ACTIVITY OF CISSUS QUADRANGULARIS LINN.

Mrs. S. Shamina*, Ms. Anjana and Dr. S. Suja

Abstract

Cissus quadrangularis Linn. is considered to be one of the important herbal plants in traditional systems. It has medicinal properties with various activities like anti-inflammatory, antioxidant, free radical scavenging, and anti-osteoporosis. Soxhlet method was used for extraction of the medicinal plant using various solvents such as aqueous, ethanol, methanol, chloroform and petroleum ether. The preliminary phytochemical screening carried out to determine the various bioactive compounds present in the different solvents of the plant extracts. The methanolic extract showed the maximum bioactive compounds among the various solvent extracts. The plant extract was evaluated for anti collagenase and anti elastase activity. The plant extract was prepared in the concentration of 100-1000μg and the in vitro anti collagenase and anti elastase assay were evaluated, piroxicam was used as positive control. The anti collagenase assay the plant extract shows the percentage of inhibition as 17.94, 26.92, 50.64, 55.12, and 64.10%. Whereas, the anti elastase assay shows the percentage of inhibition as 20.12, 32.31, 45.12, 52.04 and 57.31% respectively. The results from the anti collagenase and anti elastase assay shows the plant extract has a vibrant ability of protecting the degradation of connective tissue proteins, it includes potential like anti-aging and anti-arthritic activity which can be approved with the presence of the bioactive compounds like alkaloids, phenols, tannins from the phytochemical screening of the plant.

Keywords: Cissus quadrangularis, anti-collangenase, anti-elastase, methanol, phytochemical screening, Soxhlet method.