Abstract

SIMULTANEOUS SPECTROPHOTOMETRIC ESTIMATION OF CINNARIZINE IN BINARY MIXTURE WITH PIRACETAM IN BULK AND PHARMACEUTICAL DOSAGE FORMS

Ahmed MS Al-Ghani*, Anes A. M. Thabit and Nabil Albaser

Abstract

Cinnarizine, an antihistaminic drug, is commonly formulated in combination with piracetam for treat wide range of disease as dizziness, nausea, inner ear syndrome and other diseases of the brain. The aim of this work was to develop simple, precise and accurate spectrophotometric method for the estimation of Cinnarizine (CIN) in binary mixture with piracetam (PRM) in bulk and Formulated dosage form. This method could be used in routine analysis in laboratories of quality control. Two chemometric techniques include principal component regression (PCR) and partial least squares (PLS) were prepared using synthetic mixture containing two drugs, all dissolved in methanol. In PCR and PLS the absorbance of the synthetic mixture in the range 218-230 nm with interval Δλ=0.5 nm in their zero-order spectra were selected. Calibration or regression was then obtained by using the absorbance data matrix and the concentration data matrix for the determination of the unknown concentrations of CIN and PRM in laboratory prepared mixtures and pharmaceutical dosage form. The two proposed technique were positively applied for analysis of the two drugs in laboratory prepared mixture and in dosage form with good recoveries percent in the range of 98-102%. Method validation was conducted according to ICH guidelines. The developed methods are simple and precise and can be used for routine analysis of the drugs in combined dosage forms in quality control laboratories.

Keywords: Cinnarizine; Chemometric; Partial least squares; Piracetam; Principal component regression; Validation.