Abstract

ROSIGLITAZONE NANOPARTICLES LOADED IN HYDROGEL FOR THE ACCELERATED HEALING OF FULL THICKNESS EXCISION WOUNDS IN DIABETIC RATS

Mohammed Tajuddin, Rakesh Kumar Jat and Nagakanyaka Devi Paladugu*

Abstract

Diabetic wound (DW) is a major problem which often requires amputation of the concerned organ in diabeticpatients. In diabetes, the prolonged inflammation phase hinders the further phases of healing which, in turn, lead to improper healing of the wounds. Rosiglitazone (Ros) is an anti-diabetic drug with reported anti-inflammatory properties. The aim of the current study is to develop a Ros-chitosan nanoparticle (Ros-CS-NPs) loaded collagen hydrogel and evaluate its healing ability in DWs. The results of characterization of hydrogel reveal that the ROS-CS-NPs loaded hydrogel possess optimum particle size, controlled Ros release, biocompatibility and enhanced the cell migration. Using the streptozotocin and high fat induced DW model, significantly (p<0.05) faster rates of wound contraction in ROS-CSNPs loaded hydrogel treated group was observed in comparison with that in control and CSNPs loaded hydrogel treated groups. The ELISA results indicate a significant (p<0.05) decrease of MMP-9 levels in the ROS-CS-NPs loaded hydrogel treated group as compared to those in control groups. Thus, the use of ROS-CS-NPs loaded hydrogel can prove to be a capable approach in the treatment of DWs.

Keywords: Rosiglitazone; Chitosan nanoparticles; Collagen; Hydrogel and Diabetic wound.