Abstract

RESTORATIVE EFFECT OF WITHANIA SOMNIFERA AT CELLULAR AND SUBCELLULAR LEVELS IN CARDIOMYOCYTES AGAINST ENDOSULFAN INDUCED TOXICITY IN SWISS ALBINO MICE

Sanjeev kumar jha*, Amita kumari and Dilip kumar paul

Abstract

India being an agriculture-based country is dependent on cultivation of crops at mass scale. Hence, pesticides are extensively utilized for the better yield of crops. Endosulfan is an organochlorine pesticide, widely used by the farmers of India. In the recent times it has caused serious health hazards in the exposed population. The cardiovascular diseases have also flared up many folds in the exposed population. Hence, the present research work aims to observe the restorative effect of Withania somnifera against Endosulfan induced cardiotoxicity at cellular and subcellular levels in Swiss albino mice. Endosulfan at the dose of 3mg/Kg body weight per day was administered orally to Swiss albino mice for 4 weeks. Then after, Withania somnifera at the dose of 1000 mg/Kg b.w was orally administered for 4 weeks. Mice were sacrificed after the completion of the entire treatment. After dissection the tissue samples were collected fixed in respective fixatives for light microscopic study as well as Electron microscopic study. The study shows deleterious effect of Endosulfan at cellular and subcellular levels. But, after administration of Withania somnifera there was significant normalisation in the cardiomycytes at the cellular as well as cellular levels. Therefore, from the entire study it can be concluded that Withania somnifera plays the vital role to control the endosulfan induced cardio-toxicity at cellular as well as subcellular levels.

Keywords: Endosulfan, cardiotoxicity, Withania somnifera, restorative effect, light microscopy and Electron microscopy.