Abstract

A STUDY ON EXTENDED SPECTRUM BETA LACTAMASE PRODUCING GRAM NEGATIVE BACILLI AMONG BLOOD CULTURE ISOLATES AND THEIR ANTIBIOTIC SUSCEPTIBILITY PATTERN FROM INTENSIVE CARE UNITS IN A TERTIARY CARE HOSPITAL

Dr. Chitralekha Saikumar* and Dr. M. Nishanthy

Abstract

Infection rates in ICU have been found to be high among hospital acquired infections. Blood stream infections lead to higher mortality and morbidity. This study was done to determine the prevalence of Extended spectrum beta lactamase producing gram negative bacilli among blood culture isolates from ICU and their trends of antibiotic susceptibility patterns which is prevalent among these isolates in our Institute from ICU patients. The study was conducted in the Department of Microbiology at Sree Balaji Medical College and Hospital from December 2017 to December 2018.Total number of blood samples received were 1440 in our microbiology laboratory from ICU. Isolates were identified by colony morphology, gram stain and standard biochemical reactions. Antibiogram was determined by Clinical and Laboratory Standards Institute (CLSI) guidelines. Total positive culture present in the study 156(10.8%). Among these culture positive isolates 106 were gram positive (67.95%) isolates and 50 were gram negative (32.05%). The most common isolate was CONS followed by E.Coli, Klebsiella pneumoniae, Klebsiella oxytoca, Proteus vulgaris, Pseudomonas aeruguinosa, Acinetobacter baumanni species. Most of the gram positive isolates were susceptible to Linezolid, vancomycin and clindamycin. All the extended spectrum beta lactamase producing gram negative isolates showed 100% susceptibility to Carbapenems, Colistin and Polymyxin-B.

Keywords: Blood stream infections, Intensive care unit, Antimicrobial resistance, Coagulase negative Staphylococcus, E.Coli.