Abstract

QUANTITATIVE ANALYSIS OF CASIEN BY PRECIPITATION FROM THE VARIOUS MILK POWDER SAMPLES & DETECTION OF METALS IN MILK POWDER SAMPLES

*Dr. Anita P. Patil

Abstract

The present research deals with the separation of casein from milk samples from various commercial milk samples of cow, buffalo and goat milk. The milk samples were analysed in laboratory for casein estimation by commercial precipitation method. Further the samples were further processed for inorganic qualitative analysis to investigate the various metals present in the milk samples by routine chemical analysis. After qualitative analysis of these milk samples, these three samples viz. cow, buffalo and goat milk samples were investigated by Energy Dispersion Spectroscopy (EDS) technique, to get the exact composition of metals present among these samples. From EDAX analysis it was observed that the cow, buffalo and goat milk samples contain large of transition metals in various concentration. The transition metals observed in these samples are Cr, Mn, Ni, Fe, Zn, Co, Cu & Cd. Further more the qualitative investigation of milk samples was investigated to find out the casein present in the various milk samples. From this investigation it was found that the cow milk samples contain 7.8 g of casein, the buffalo milk sample contains 4 g and goat milk sample contains 6.4 g of casein. From overall comparison it can be seen that cow milk sample contains more percentage of casein in contrast to buffalo and goat samples.

Keywords: Milk powder, casein, EDS, qualitative and quantitative estimation.