Abstract

IN-VITRO STUDY OF FORMULATION AND EVALUATION OF GLIMEPIRIDE NANOSUSPENSION BY PRECIPITATION METHOD

*Soumya M.

Abstract

In the present study, an attempt was made to prepare oral Nanosuspensions of glimepiride is the first III generation sulphonyl urea it is a very potent sulphonyl urea with long duration of action. Glimepiride is indicated to treat type 2 diabetes mellitus; its mode of action is to increase insulin production by the pancreas in order to overcome bioavailability problems, to reduce dose dependent side effects and frequency of administration. The drug is well absorbed from gastrointestinal tract, but its bioavailability is low due to extensive first pass metabolism Nanosuspension containing the drug was prepared by precipitation method using combinations of polymers (such as PVP-k25, Urea, Acetone, SLS, and Poloxamer F127). Estimation of glimepiride was carried out spectrophotometrically at 221 nm. The Oral Nano suspension were evaluated for various physical and biological parameters, drug content uniformity, entrapment efficiency, scanning electron microscopy, in-vitro drug release, short-term stability, and drug-excipient interactions (FTIR) indicates that all were within limits. In-vitro drug release studies of all the formulations were studied of that formulation F1 shows 99.74% of drug release within 30 min and follows first order release kinetics. IR spectroscopic studies indicated that there are no drug-excipient interactions.

Keywords: Glimepiride, Oral Nanosuspensions, Urea, PVP-k25, SLS, Poloxamer F127 and Acetone.