Abstract

ACUTE AND REPEAT DOSE TOXICITY ASSESSMENT OF PROTEIN SUPPLEMENT (NRL2019NMP) - IN MICE

Priya Khare, Dr. Omkar Kulkarni*, Dr. Anuj Agrawal, Dr. Gayatri Ganu and Dr. Sohan Chitlange

Abstract

The body requires many different vitamins and minerals that are crucial for both body development and preventing disease. A nutritional deficiency occurs when the body doesn’t absorb or get from food the necessary amount of a nutrient. Deficiencies affect health and wellbeing. So it is very important consideration of any nutritional supplement to undergo toxicological analysis. The aim of this study was to evaluated safety profile of the NRL2019NMP, a protein supplement with micronutrients fortification using acute and repeat dose toxicity studies in Mice model. The acute toxicity test revealed no observable signs of toxicity mortality or morbidity at 5000mg/kg. There were no significant variations in the hematological, biochemical, clinical, histopathological parameters of both NRL2019NMP treated and control group mice during 28 days study. Based on the findings of this study, the no observed adverse effect level (NOAEL) of NRL2019NMP in mice, following oral administration for 28 days was found to be 1000 mg/kg body weight.

Keywords: histopathology, acute toxicity, repeats dose toxicity.