Abstract

IN VITRO ANALYSIS OF ANTI- ADVANCED GLYCATION EFFECT OF POLYHERBAL COMPOUND TRIPHALADI COMPOUND CAPSULE AND ELANEER KUZHAMBU ANJANA

Dr. Hitesh Bhati*, Dr. Dharmendra Singh Vaghela and Prof. Rajgopala Manjusha

Abstract

Background- Non-enzymatic or Advanced glycation between reducing sugar and protein results in the formation of fluorescent and insoluble advanced glycation end products (AGEs), which is believed to play important roles in the pathogenesis of diabetic and aging complications. Objectives- To determine anti- advanced glycation activity of an Anubhuta (experienced) polyherbal formulation Triphaladi Compound capsule and Elaneer Kuzhambu Anjana a semisolid drug of eye drop mentioned in Ayurvedic text Sahastrayoga, to the reference Arbutin a known inhibitor of glycation reaction by using an in vitro glucosebovine serum albumin (BSA) assay. Material and Method- Glucose and BSA were incubated at 60°C in the presence and absence of drug samples. Following a 24-hour incubation period, the Glycated BSA product was precipitated with Trichloroacetic acid (TCA) and redissolved in alkaline phosphate buffered saline (PBS). In spite of the sample, the phosphate buffer saline was used as the sample control and Arbutus was used as the reference standard. Negative control was carried out at the same time with BSA, phosphate buffer saline and drug sample incubated under the same conditions. The formation of Glycated BSA was relatively quantified based on fluoresce intensity by measuring fluorescence intensity. Excitation and emission wavelengths were at 370 nm and 440 nm, respectively. Each sample was analyzed in triplicate form. Results- Triphaladi Compound capsule and Elaneer Kuzhambu Anjana showed a significant increase in anti advanced glycation activity with an increase in their concentration. Conclusion- The present study showed that the Triphaladi compound capsule and Elaneer Kuzhambu Anjana as an anti-glycation agent may prove beneficial in diabetic and aging complications.

Keywords: Elaneer Kuzhambu Anjana; Triphaladi compound capsule; Arbutin; advanced glycation end product; glucose-BSA assay.