Abstract

STUDY ON RADICAL SCAVENGING AND GASTROPROTECTIVE EFFECTS OF ETHANOLIC STEM-BARK EXTRACT OF CARPOLOBIA LUTEA IN RODENTS

Lucky Legbosi Nwidu*, Jonah Sydney Aprioku, Fred Horsfal Guluye

Abstract

This is a study of the antioxidant activity and gastroprotective effects of Carpolobia lutea ethanolic stem-bark extract (CL ESE) used as antiulcer in ethnomedicine. Experimentally induced gastric ulceration was achieved using acute models such as indomethacin, ethanol, stress and serotonin. Amino acid analysis was by cation-exchange chromatography using automated amino acid analyser. Antioxidant potential was obtained by spectrophotometric assay using 2, 2- Diphenyl-1-picrylhydrazyl DPPH. The finger print of ESE was revealed by Jasco (Tokyo, Japan) HPLC and active compounds by phytochemical screening using standard procedure. The calculated LD50 is 866.025 mg/kg (i.p) using Lock's Method. In the indomethacin model, 43.3, 86.6 and 173.2 mg/kg revealed ulcer inhibition rate of 51 %, 65 % and 76 % respectively (p > 0.05-0.01) when compared with the control (p

Keywords: Carpolobia lutea, stem-bark extract, antiulcerogenic, amino acid and antioxidant profile.