Abstract

GCMS ANALYSIS AND ANTIBACTERIAL ACTIVITY OF COCOS NUCIFERA AND MUSA ACUMINATA AGAINST MULTI DRUG RESISTANT ESCHERICHIA COLI AND STAPHYLOCOCCUS AURESUS

I. M. Agnihotri*, S. S. Chari and D. G. Kadam

Abstract

The present study was undertaken to determine antibacterial activity of Cocos nucifera and Musa acuminata leaf extracts against multi drug resistant Staphylococcus aureus and Escherichia coli. The leaf extracts of C. nucifera and M. acuminata were prepared by Continuous Soxhlet extraction and cold extraction method. Antibacterial activity was done by agar well diffusion method and MIC values were determined by using macro-broth dilution method. Antibacterial activity present in both the extracts; methanol extract of Musa acuminata and Cocos nucifera showed more antibacterial activity against multi drug resistant Staphylococcus aureus followed by the methanol extract of Cocos nucifera against Escherichia coli. GCMS analysis of both plant extracts showed presence of biologically active compounds 6- Octadecenoic acid, methyl ester, (Z)-, Neophytadiene and Squalene that possess pharmacologically potential activity. The Cocos nucifera and Musa acuminate leaf extracts may have therapeutic value in control of certain multidrug resistant bacterial pathogens.

Keywords: Antibacterial activity, GCMS analysis, Cocos nucifera, Musa acuminata, Staphylococcus aureus, Escherichia coli.