Abstract

EVALUATION OF ANTIOXIDANT ACTIVITY, TOXICITY STUDY AND STANDARDIZATION OF TCCT TABLET

Vidyadhar Vaidya*

Abstract

Background and objective: Oxidative stress is a common factor for various diseases like cancer, diabetes, metabolic disorders, atherosclerosis, and cardiovascular diseases. The incidence of such diseases is growing day by day around the globe and causes various complications. The disease not only affects the quality of life but also the mortality rate with these diseases are too high. Considering these factors, it is important to come up with a simple yet potential solution for reducing oxidative stress. Therefore, RV New Visions Health Care Pvt. Ltd. has developed Turmeric and Cinnamon chewable tablets (TCCT) as a potential treatment for reducing oxidative stress and fighting cancer. Materials and Methods: Antioxidant activity of Turmeric and Cinnamon chewable tablet was evaluated In-vitro by using DPPH scavenging assay, H2O2 radical scavenging assay, and reducing power assay. Further, toxicity testing in animal models and the standardization using the HPLC and HPTLC method was done. Results: The formulation Turmeric and Cinnamon chewable tablet showed significant anti-oxidant action when compared with standard ascorbic acid. The percentage scavenging activity at 50 - 500 ug/ml, 14.2 - 53.55 % by DPPH scavenging assay, 22.26 – 50.81 % by hydrogen peroxide scavenging assay. The reducing power was found to be increasing with increasing absorbance in the reducing power assay. Conclusion: The study thus proves, Turmeric and Cinnamon chewable tablet has significant antioxidant activity

Keywords: Oxidative stress; Antioxidant; Cancer; Standardization; Herbal formulation; Toxicity testing.