Abstract

PHYSICOCHEMICAL ANALYSIS AND STANDARDIZATION OF ANDROGRAPHOLIDE AND GALLIC ACID BY RP-HPLC IN VILOCYM PREMIX: A HERBAL VETERINARY FORMULATION

Ravikanth Kotagiri, Anirudh Sharma, Pushap Lata* and Deepak Thakur

Abstract

Background: Vilocym Premix, a polyherbal veterinary formulation of Ayurvet Limited, Baddi, is a multiple mycotoxin binder, immunomodulator and growth promoter for use across species, containing herbs like Andrographis paniculata, Terminalia chebula, Phyllanthus emblica and Solanum nigrum as the major ingredients. Objective: The present study was undertaken to develop standardization parameters for Vilocym Premix. Evaluation of various standardization parameters like organoleptic characters, physicochemical evaluation and estimation of bioactive markers Andrographolide and Gallic acid by reverse phase high performance liquid chromatography (RP-HPLC). Material and method: Vilocym Premix was obtained from the QA/QC department of Ayurvet Limited Baddi. Andrographolide and gallic acid were used as standards. Standards and sample dilutions were prepared as per Standard Test Procedure. Vilocym Premix was evaluated for its organoleptic and physicochemical properties, RP-HPLC analysis was done using mobile phases acetonitrile: water (0.1% orthophosphoric acid):: 40:60 for andrographolide and acetonitrile: water :: 20:80 (pH were adjusted to 3 with orthophosphoric acid) for gallic acid. Results: The RP-HPLC chromatograms of Vilocym Premix, showed standard andrographolide and gallic acid at retention time of 4.83 min and 3.60 min. The percentage of andrographolide was 0.097 % w/w and the percentage of gallic acid was 0.071 % w/w in Vilocym Premix formulation. Further, data from the physicochemical and organoleptic study of the formulation added the quality optimization of the product. Conclusion: The characterization parameters presented in this communication shall serve as a standard reference for quality control analysis of Vilocym Premix for its batch to batch consistency.

Keywords: RP-HPLC, phytoconstituents, andrographolide, gallic acid, standardization, Vilocym Premix.