Abstract

DETECTION OF GENTAMYCIN RESIDUE LEVELS IN MASTIC COW’S MILK FOLLOWING INTRAMAMMARY ADMINISTRATIONS

Samia A. A. Hassan, Sania A. I. Shaddad* and Huda O. Ali

Abstract

The excessive use of antimicrobial drugs in dairy farm may result in the presence of their residues in milk and milk products, which can adversely affect public health. This study was conducted to determine the presence of gentamycin residues in cow Milk samples after treated with intramammary infusion for mastitis. Information about treatment and drugs were estimated by questionnaires and direct interview with farm’s owner. A total of 300 milk samples was collected during the period from September 2018 to October 2019 of 200 farms in Khartoum north (100 samples), Khartoum (100) and Elgazeera state (100) samples for 5 days after the last administration. The milk samples were screened for gentamycin residues using Delvo®-test and a modifi one plate test (O.P.T.) using Bacillus subtilis (BGA-DSM-618) as a test organism. The result indicated that out of 300 milk samples were positive for antibiotic residues (83%) of these were in Egazeera (69%) were in Khartoum north and lowest percentage were in Khartoum (39%). To obtain the gentamycin concentrations the inhibition zones diameter were interpreted with gentamycin standard curve. Gentamycin levels were evaluated in different times. Highest levels were noted at first day while the lowest were in fifth day after withdrawal period which were greater than its international maximum residues limit (MRL).

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