Abstract

PHARMACOKINETICES AND TISSUE RESIDUES OF TETRACYCLINE IN CHICKENS FOLLOWING ORAL ADMINISTRATION

Samia A. A. Hassan, Selma O. A. and Sania I. A. Shaddad*

Abstract

Tetracyclines are one of the antibiotics classes that have been extensively used in poultry farms in therapeutic doses for treatment and prevention of bacterial infections and in sub-therapeutic doses for growth promotion. This may result in the presence of their residues in edible tissues intended for human consumption, causing a health threat. Therefore, this study was conducted to obtain the pharmacokinetics parameters of tetracycline in broiler chickens and tissue residues. The pharmacokinetics profile was studied after oral administration at dose of 70mg/kg bodyweight. Plasma concentrations were determined using modified microbiological assay method. The inhibition zones diameters were interpreted with tetracycline standard curve to determine plasma and tissue concentrations. A peak level of tetracycline Cmax 41.30±6.20 mg/μl was achieved after tmax of 0.9±0.15h and half-life (t½ β) was 11.51±3.86h and the volume of distribution Vd was found to be 0.48±0.13L/kg. the total clearance (Cl β) was 0.93±0.16μl-1 and the mean resident time was 10.43±4.83. A total of 150 tissue samples (Liver, kidney, muscle) was screened for detection of residues after multiple doses of 70 mg/kg body weight of tetracycline administered orally for 5 days. Tissue samples were collected at 1, 3, 7, 10 and 12 days after drug administration. The samples were processed using microbiological assay method. The highest concentrations were detected 1286.76 ±375.13μg/g, 848.265±135.082 and 9341.62±420.51 in liver, kidney and muscle respectively at day 1 while day 12 showed significant lower concentrations of tetracycline above maximum residue limits (MRL).

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