Abstract

CHROMATOGRAPHIC FINGERPRINT ANALYSIS OF STEROLS IN OROXYLUM INDICUM (L) VENT BY HPTLC TECHNIQUE

Saraf Aparna* K. Srilatha Srinivas

Abstract

The study was carried out with an aim to determine the chemicalprofile of sterols from leaf , stem and roots of the medicinallyimportant plant Oroxylum indicum (L) Vent collected from twodifferent geographical regions of India viz., Western Ghats and NorthEast. Preliminary phytochemical screening was done followed byextraction of sterols and then by HPTLC studies. Toluene: methanol(9:1) was used as mobile phase for the separation of sterols. βSitosterol was used as a marker compounds and was detected in all theplant parts collected from both the regions. A good seperation ofsterols was observed at 540nm. The study revealed diverse forms ofsterols in large number in the root, stem and leaf of Oroxylum indicum (L) Vent. from boththe regions. It can be concluded that HPTLC fingerprint analysis of root, stem and leafextract of Oroxylum indicum (L) Vent can be used as a diagnostic tool for the correctidentification of the plant and it is useful as a phytochemical marker. These separated sterolsin addition of being developed directly as drugs can also served as prototype drug moleculesknown as “Lead Compounds” and as pharmacological probes to help better understandbiochemical and physiological mechanisms.

Keywords: Oroxylum indicum (L) Vent, sterols, HPTLC fingerprint.