Abstract

PHYSICOCHEMICAL ANALYSIS AND ANTIBACTERIAL ACTIVITY OF CASCABELA THEVETIA (L) LIPPOLD SEEDS

*Sandhya Bhoyar

Abstract

The recent study reveals that the phytochemical investigation, physicochemical analysis and the antibacterial potential of seeds of plant Cascabela thevetia. It includes the parameters like moisture content, ash value, total ash, acid insoluble and acid soluble ash values from the seeds of C. thevetia. The seed extracts were also screened for its antibacterial potential. The seeds are extracted with five different polar solvents such as petroleum ether, ethanol, methanol, acetone and distilled water. Such extracts were subjected for phytochemical screening. The qualitative analysis of all the extracts shows the presence of phytochemicals like alkaloids, flavonoids, tannins, steroids, carbohydrates and amino acids. The physicochemical analysis of seeds of Cascabela thevetia showed (4.26%) moisture content, total ash (8.9%), acid insoluble (4.8%), water soluble ash (5.1%), alcohol soluble extractives (20%) and water soluble extractives (29.6%). The different extracts subjected for antibacterial activity against six bacterial strains like E. coli, S. typhi, K. pneumonae, P. aureginosa, S. aureus and Sh. flexinari. The results shows the maximum inhibition of seed extract against K. pneumonae (38 mm) in dist. Water and P. aureginosa (29 mm), Sh. flexinari (29 mm) and S. aureus (28 mm). in acetone.

Keywords: Physicochemical analysis, phytochemical screening, antibacterial assay, Cascabela thevetia.