Abstract

PREPARATION AND CHARACTERISATION OF CAPECITABINE LOADED PLGA NANOPARTICLES FOR COLORECTAL CANCER

Prasanna S.Sutar*, Vijaya G. Joshi, Kishori P. Sutar, Kishore G.Bhat

Abstract

The main goal of the present investigation was to formulate and evaluate Capecitabine loaded PLGA nanoparticles for colon cancer treatment. Nanoparticles of Capecitabine were prepared by Emulsion droplet coalescence method using PLGA 50-50 as the polymer. The nanoparticles obtained were evaluated for morphology, loading efficiency, in vitro release study. C2 formulation showed drug release of 66.14 at 24 hours with smaller particle size of 141.66 ± 10.23 and 61% drug loading. Concentration of PLGA 50-50 and drug release was proportionate to each other. DSC and FTIR studies showed that the drug and polymers were compatible with each other and did not reveal any signs of interactions. SEM image of optimized formulation C2 showed spherical and smooth surfaced particles. Biological evaluation was carried by using HT29 (Human colorectal Adenocarcinoma) cell lines and formulation C2 showed 60% cell lysis. This drug delivery targeted the colon cancer cells and provided a better therapy to the patient by reducing toxicity to the normal cells. Kinetic study indicated that the formulations followed first order kinetics.

Keywords: Nanoparticles, Capecitabine, Colorectal cancer, PLGA.