Abstract

PHOSPHOTUNGSTICACID (H3PW12O40) CATALYZED ECOFRIENDLY SYNTHESIS OF SUBSTITUTED COUMARINS AND STUDY THEIR ANTI-MICROBIAL ACTIVITIES

Anna Regini Anthony*

Abstract

The Chemists all over the globe are motivated not only for the environmentally benign synthesis of new products but also to develop green synthesis for existing chemicals The Coumarine heterocyclic ring is common feature of various bioactive compounds such as calanolides, lipid-lowering agents. Recent studies have been revealed that coumarin and the derivatives exhibit several other medicinal applications such as anti-coagulants, antifungal, insecticidal, hypnoticsphytoalexins, HIV protease & inhibitors. Coumarins act as in intermediate for the synthesis of various biologically active molecule such as coumarones, and fluorocoumarins. Thus the synthesis of coumarins is of continuing interest. Phosphotungsticacid (H3PW12O40) a commercially available environmentally benign catalyst non-toxic widely used for the synthesis of the substituted coumarin. Owing to the numerous advantages associated with cheap and non-hazardous catalyst, and also realizing g the importance of coumarin herein we would like to focus the eco –friendly method for his synthesis of derivatives of coumarin using cheaper and commercially available acid catalyst Phosphotungsticacid (H3PW12O40). and also by the Knoevenagel condensation under microwave irradiation. The synthesized coumarin derivatives were screened in Vitro anti-microbial efficacy testing. In vitro antimicrobial efficacy testing was carried out by broth dilution method by broth dilution method as mentioned in “Pharmaceutical Microbiology”. For anti-bacterial activity, Muller Hintonmedium was used as the nutrient media. Test bacterial species used are Escherichia coli, (ATCC 10148), Staphylococcus aureus(NCTC 3750), Pseudomonas aeruginosa (Fisher‟ Immunotype IV), test fungi species used are Aspergilliusniger(ATCC 16404) and Candida albicans (ATCC 10231) in different concentrations starting from 25ppm. All the coumarin derivatives are active against the test bacteria and fungai in different concentrations. This paper focuses is to develop environment friendly reactions, simple, highly efficient and high yielding protocol for the synthesis of coumarin derivatives using Phosphotungsticacid as a catalyst.

Keywords: Phosphotungsticacid, microwave irradiation, anti-microbial.