Abstract

EVALUATION OF IN-VITRO AND IN-VIVO IMMUNOMODULATORY ACTIVITY OF AQUEOUS AND ETHANOLIC EXTRACT OF BIXA ORELLANA (L.).

Pallavi Maruti Patil*, Vanita G. Kanase and Jignyasha Amit Kumar Raval

Abstract

Objective: The aim of this study is to evaluate if Bixa orellana Linn. (Bixaceae) leaves have Immunomodulatory properties. Methods: Dried leaf powder was extracted in series of solvents, including Ethanolic Extract, chloroform, ethyl acetate, methanol, and water, and extracts were tested for Immunomodulatory effects in Swiss albino mice. Acute oral toxicity investigations have been completed, and animal has been shown to be alive for 24 hours at doses of up to 1000 mg/kg. Using plethysmometer, volume of hind paw is measured before and after inflammation is induced. Level of inflammation in untreated and test drug-treated mice was assessed. Results: Ethanolic Extract demonstrated most efficacies at dosage level of 250 mg/kg after 2 hours, according to findings. When compared to conventional medicine as vehicle and control for extract, if inflammation in treated animals is less than that in untreated animals, medicine is regarded to have Immunomodulatory action. Tail immersion method was used to assess analgesic efficacy of different extracts of Bixa orellana leaves. Results showed that methanolic extract (12.4 sec) had highest action at dosage of 500 mg/kg 120 minutes after delivery.

Keywords: Bixa orellana Linn, In-Vivo and In-Vitro, Ethanol Extracts, Immunomodulatory Activity.