Abstract

IN VIVO EVALUATION OF SUN LIGHT PROTECTION PROPERTY OF ALOEVERA FORMULATIONS BY DNA FRACTIONATION TECHNIQUE

*V Malarvizhi, C Vijaya, Sudhakar Sekar, Vasudeva Rao

Abstract

Pure Aloe vera extract has a mild to severe skin irritant property depending on the type of skin if it is formulated as film forming lotion the chance of skin irritation may be less. In the present study Aloe vera sunscreen formulations were prepared by incorporating 10% of Aloe vera aqueous extract in film forming lotion and gel. In order to develop a lotion, a film forming polymer (Eudragit RS 100) at 3 levels 10%, 20% and 40% solution in iso propylalcohol with vanishing cream base was used. The consistency and spreadability were checked. Based on the results, Eudragit 10% contained formulation was very suitable for applying on the skin. The Aloe vera gel was prepared by using Carbopol 934, Sodium hydroxide, Aloe vera extract and Water. In-vivo studies were conducted in Swiss Albino mice to find out the sun protective property of the formulation. The study was carried out for 5 days by exposing the skin to UVB for 15 min in each day. UV light induced DNA damage, evidenced by isolated skin epidermal DNA (genomic DNA) and fractionation of genomic DNA carried out by DNA-agarose gel electrophoresis. DNA fractionation study shows that lotion treated groups of animal genomic DNA was not damaged by UV-B radiation. Furthermore, DNA concentration estimated by UV spectroscopy reveals that lotion treated animal groups epidermal DNA concentration level is more when compared to gel treated groups.

Keywords: Aloevera, Gel, lotion, DNA Fractionation, In-Vivo.