Abstract

PHYTOCHEMICAL SCREENING, THIN LAYER CHROMATOGRAPHY AND ANTIMICROBIAL STUDY OF RANDIA SPINOSA FRUITS

Kavita G. Agre*, Dr. Renuka J. Das and Dr. Milind J. Umekar

Abstract

The present study was aimed to carry out preliminary phytochemical screening, thin layer chromatography and antimicrobial activity of Petroleum ether and ethyl acetate extract of Randia Spinosa fruits. The plant has been reported to be used traditionally for the treatment of fever, rheumatoid arthritis and CNS depressant. The fruits were collected, shade dried and pulverized into coarse powder. It was then extracted with petroleum ether and ethyl acetate solvent by soxhlation. The extract was subjected to preliminary phytochemical screening to detect primary and secondary metabolites. It showed the presence of alkaloids, tannins, terpenoids, saponins and carbohydrates. TLC profile of petroleum ether and ethyl acetate extract was carried out using Toluene: ethyl acetate: formic acid (7:3:0.3) solvent system found to be separated with Rf values 0.75 for petroleum ether and 0.217, 0.434 for ethyl acetate extract. The antimicrobial activity showed the positive zone of inhibition for petroleum ether and ethyl acetate extract of Randia spinosa fruits.

Keywords: Randia Spinosa, petroleum ether, ethyl acetate, phytochemical screening, TLC, antimicrobial study.