Abstract

TOXICOKINETICS AND IMMUNOTOXICITY STUDY OF LAMBDACYHALOTHRIN IN WISTAR ALBINO RATS

Bhoopendra Kumar*, Nitesh Kumar

Abstract

The toxicokinetics study of lambda-cyhalothrin and effect on immune response in lambda-cyhalothrin treated wistar albino rats was conducted at single oral dose 20 mg kg−1 and 10 mg kg−1. The lambdacyhalothrin was administered in overnight fasting rats by mixing in 0.1% Tween 80 and Serial blood samples were collected at 0, 0.25, 0.5, 1.0, 2.0, 4.0, 8.0, 16.0 and 24.0 hr after administration. Liver, kidney, heart, adrenal, spleen, brain, testis and ovaries samples were also collected at same time interval. The concentration of lambdacyhalothrin concentrations were determined by HPLC. The humoral immune response and cellular immune was observed. The plasma and tissue concentration–time data for lambd-acyhalothrin were found to fit a one-compartment open model. The elimination half-life and mean residence time for lambda-cyhalothrin were found 5.09±0.16 h and 7.32±0.11 h, respectively. The mean volume of distribution (Vdarea) was calculated to be 3.94±0.12 L/kg. The total body clearance (ClB) was observed to be 0.54±0.01 L.kg-1.h-1. The maximum plasma concentration of lambda-cyhalothrin (Cmax) was noted to be 6.42±0.11 μg.ml-1 at tmax of 2.00 ± 0.00 h exhibit that after oral administration, lambda-cyhalothrin was extensively but slowly absorbed. The maximum concentrations Cmax was observed highest in spleen (17.9±0.38), ovaries (9.77±0.81), liver (7.75±0.28), kidney (7.75±0.28), heart (4.90±0.42), adrenal (4.87±0.69), brain (6.63±0.25) and testis (1.46±0.10) μg.ml-1. Tube agglutination test results showed significant (p<0.05) inhibition of antibody titre against S. typhimurium „O‟ antigen. The cellular immune response ex-vivo and in-vitro effects of lambda-cyhalothrin on splenoc- proliferation significant (p<0.05) decreased.

Keywords: Lambda-cyhalothrin; toxicokinetics; body organs; tube agglutination test.