Abstract

THE EFFECT OF SOME SELECTED CHEMICALS ON STREPTOCOCCUS MUTANS FROM DENTAL CARIES ONTO DENTURES AND AMALGAMS.

Suzan S. Husain*, Saadi M. D. Al-Nuzal, and Haibat Maher Abbas

Abstract

In the present study 13 isolates of Streptococcus mutans were collected from 25 volunteers within the ages of 10 - 30 years. Diagnosis was obtained according to the colony of morphology, Gram stain, biochemical test and Vitek system. They found to be 100 % sensitive to azithromycin by disk diffusion method (Kirby Bauer test). It showed sensitivities against some selected chemicals such as zinc oxide nanoparticals, phenytoin B.P, potassium L-proline dithiocarbamate, potassium oxalic acid dihydrazide bisdithiocarbamate, potassium succinic acid dihydrazide bisdithiocarbamate, and potassium adipic acid dihydrazide bisdithiocarbamate, and adipic acid dihydrazide bisdithiocarbamate by well diffusion and broth dilution methods. Azithromycin showed very strong antibacterial effect, when mixed with the amalgam components at an amount of 0.8 to 25 mg per capsule, while oxalic acid dihydrazide bisdithiocarbamate showed antibacterial effect at an amount higher than 10 mg per capsule. The other selected chemicals showed 100 % sensitivities, while L-proline, oxalic acid dihydrazide, succinic acid dihydrazide, adipic acid dihydrazide showed no antibacterial activity. The measured MIC and MBC of the selected chemicals showed the following values: Zinc oxide NPs, 5, 10 mg/mL; phenytoin B.P, 1 × 10-3, 2 × 10-3; potassium L-proline dithiocarbamate, 2 × 10-3, 2 × 10-3; potassium oxalic acid dihydrazide bisdithiocarbamate, 0.5 × 10-3, 1 × 10-3; potassium succinic acid dihydrazide bisthiocarbomate, 1 × 10-3, 2 × 10-3, potassium adipic acid dihydrazide bisdithiocarbamate, 1 × 10-3, 2 × 10-3 respectively. The measured MIC and MBC of the selected chemicals loaded on PMMA resin showed the following values: zinc oxide NPs, 30, 40 mg/mL; phenytoin B.P, 2.5, 5 mg/mL and potassium oxalic acid dihydrazide bisdithiocarbamate, 1.25, 2.5 mg/mL, respectively.

Keywords: Streptococcus mutans, azithromycin, dental caries, denture, amalgams, phenytoin B. P, dicarboxylic acid dihydrazide bisdithiocarbamate.