Abstract

BLUMEA LACERA: ANTIOXIDANT, CYTOTOXIC AND PHYTOCHEMICAL ANALYSIS OF SOLVENT EXTRACTS

A. K. Sudha Chandan and Sunita Bhatnagar*

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Abstract

In present study, the whole plant of Blumea lacera was investigated for their phytochemical, cytotoxic and antioxidant properties. Solvent extracts of the plant revealed the presence of terpenoids, flavonoids, tannin and sapponins in a number of extracts. Cytotoxic property of the sample was done using Brine shrimp lethality bioassay where hexane and chloroform showed significant activity (100%) at a highest dose of 200microgram/ml. Antioxidant activity was conducted qualitatively and quantitatively both. In qualitative TLC based antioxidant assay, maximum number of antioxidant bands was obtained in chloroform extract of EMW solvent. For quantitative antioxidant analysis FRAP (Ferric reducing antioxidant power assay) and DPPH(1,1-diphenil-2-picrylhydrazyl)radical scavenging assays were explored. Methanol extracts exhibited significant activity in both the assays.

Keywords: Blumea lacera, medicinal plant, phytochemicals, cytotoxic, antioxidant, TLC, DPPH radical scavenging assay, FRAP.