Abstract

PROTECTIVE EFFECT OF POLYBION AGAINST RADIATION AND CADMIUM INDUCED BIOCHEMICAL CHANGES IN THE LIVER OF SWISS ALBINO MICE

Ramesh Purohit*, Radheshyam Joshi, Aruna Chakrawarti and R.K. Purohit

.

Abstract

The extensive use of atomic energy now a days in various branches of natural economy, science and technology, radiodiagnosis, radiotherapy, industries, agriculture, nuclear researches etc. has made radiation injury an urgent problem attracting the attention not only of specialists in a variety of clinical disciplines but also of a vast army of theoretical scientists. Metals like cadmium have always been an intrinsic components of earth crust with the continuing trends towards and increasing human activities involving man may become exposed to concentration of toxic metals presenting a potential threat for survival. The severity of the damage can be modulated by treated the animals with antioxidants. In view of the potential for practical application, a variety of compounds are being tested for their radioprotective activities. Among these, vitamins hold a great promise. Polybion is a vitamin B-complex mixture that is easily available in the market in the form of tablets, syrup, capsules and injection. The drug is prescribed in India for the treatment of various types of body dysfunctions. In light of the above, the present study was aimed to evaluate the protective effect of polybion against radiation and cadmium induced histopathological changes in the liver of Swiss albino mice. The animals from all the experimental groups were sacrificed by cervical dislocation at each post-treatment intervals of 1,2,4,7,14 and 28 days. After sacrificing the animals, pieces of the liver were taken out and some of them were immediately fixed in Bouin’s fluid for histological observations by routine procedure. The changes observed were distortion of hepatic architecture, intracellular oedema, narrower sinusoids, cytoplasmic degranulation, vacuolation, hyperaemia, pycnotic and crenated nuclei. In the combined treatment of radiation and cadmium chloride synergistic effects were observed. The liver of polybion treated animals exhibited less severe damage as compared to non-drug treated animals at all the corresponding intervals. An early and fast recovery was also noticed in polybion pretreated animals. Thus, it appears that polybion is potent enough to check cadmium and radiation induced hepatic lesions in Swiss albino mice.

Keywords: Polybion, Antioxidant, Liver, Radioprotection.