Abstract

IN VIVO STUDIES OF POLYDATIN IN PERIPHERAL NEUROPATHIC PAIN MODEL ON MALE SPRAGUE DAWLEY RATS

W. Clement Atlee*, P. Muralidharan and Kalaiselvan K.

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Abstract

Aim: Polydatin is an active ingredient from polygonum cuspidatum herb, a Chinese plant. Because available allopathic drugs have lot of side effects and unproven therapeutic benefit, polydatin like herbal drug would be a correct safer alternative to treat peripheral neuritis. Peripheral neuropathy (PN) affects peripheral nerves and causes neuropathic pain. Peripheral neuropathy may be caused by diseases like diabetes, drugs like paclitaxel or by nerve injuries. We have done this present study to explore the ameliorative action of Polydatin on peripheral neuropathy induced by paclitaxel on male Sprague dawley rats. Methods: Peripheral neuropathy was induced in Sprague dawley rats in group-II by paclitaxel (2mg/kg/2days) intra peritoneal injection (i.p) over the course of one week. Group 4 and 5 administered with Polydatin100mg/kg and 200mg/kg respectively (from 7th day onwards) orally. Group 3 rats were administered with standard drug tramadol orally. Experimental rats from groups 1 to 5 were subjected to in vivo studies like hot plate, cold plate, rorarod performance and locomotor activity and behavioral tests like food eating, water drinking, and weight checking. These values were subjected to one-way ANOVA (Analysis of Variance) statistical method followed by Dunnets‟t test to assess the significant difference between the groups and diseased (Group II). Results: Decrease in jumping and paw licking response in tail flick and cold plate methods, and increase in food intake, water intake and body weight, increased rotarod and locomotor activity in polydatin group animals were observed when compared with the disease group (group II) rats. Conclusion: Our study demonstrated the potential of polydatin in ameliorating paclitaxel-induced peripheral neuropathy.

Keywords: Peripheral neuropathy, Polydatin, Paclitaxel induced neuropathy, Neuroprotective, Polygonum cuspidatum, tramadol.