Abstract

INTERACTION OF PEPTIDE GROUPS OF PLASMIN WITH GOLD NANOPARTICLES

Pratibha Kamble*, Sameer Kulkarni Dayanand.A.Bhiwgade

Abstract

Background: Bioconjugation of nanoparticles to proteins is challenging approach as various amino acid groups of protein are involved in nucleation process for growth and establishing electrostatic interactions and stabilizing the nanoparticles. Use of Plasmin in current experiment has been thought to serve dual purpose, it acts as a reducing agent and also stabilizes gold nanoparticles. The nanoparticles formed are characterized by using TEM, UV-Vis spectroscopy, XRD and FTIR. Results: Our results of transmisssion electron microscopy (TEM) shows gold nanoparticles synthesized have average size ~20-50nm showing spherical symmetry, also the particles are all aggregated. The synthesis of particles from HAucl4 to Auplasmin conjugates detected by change in absorption spectra. The spectral signatures showed surface plasmon resosnance band at λmax 545nm indicating the formation of 10nm, 20nm and 50nm gold nanoparticles. The diffraction patteren at 38.3 corresponding to (111) planes of fcc gold phase also indicated formation of Au-plasmin conjugated gold nanoparticles. In addition FTIR spectroscopy results showed signals at 1032 cm-1, 1111 cm-1, 1559.84 cm-1, 2924.65 cm-1 indicated different groups such as COO-, C-H involved in forming electrostatic interaction and stabilization of nanoparticles. Conclusion: The application of plasmin in current study indicates that it serves as efficient means for synthesizing the nanogold. The gold nanoparticles thus formed by this method is safe, biofriendly at the sametime showed uniform spherical shape.

Keywords: Gold nanoparticles, : Gold nanoparticles, : Gold nanoparticles, : Gold nanoparticles, Plasmin, spherical, stabilization, biofriendlyPlasmin,