Abstract

AREA UNDER CURVE BY UV SPECTROPHOTOMETRIC METHOD FOR DETERMINATION CARBIMAZOLE IN BULK

Shingote Vishakha Shankar*, Bornare Snehal Laxman and S. D. Mankar

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Abstract

The aim of present investigation is to establish simple, precise, and rapid Spectrophotometric method for the quantification of Carbimazole in Active Pharmaceutical Ingredient. In this, work is carried out to for estimation of Carbimazole bulk by utilizing an Area under Curve (AUC) method using UV – Visible Spectrophotometry. The study is designed to validate the developed methods as per ICH guidelines. For this purpose the wavelength range between 200-400 nm was selected. alcohol and acetone(50 ml acetone used for stock solution and serial dilution in 25 ml distilled water) was used as a solvent throughout the work. Linearity was obtained in concentration range 2 to 10 ɥg/ml (r2 = 0.992) for the method. The developed method was found to be simple, linear, accurate, precise and highly sensitive and which can be used for routine quality control analysis for Spectrophotometric estimation of Active Pharmaceutical Ingredient.

Keywords: Carbimazole, linearity, AUC, spectrophotometer, alcohol, and Acetone.