Abstract

PHYLOGENETIC ANALYSIS OF 16SrDNA AND 18SrDNA SEQUENCES OF BACTERIA AND FUNGI FROM KERATITIS PATIENTS

Dr.V. Nithya

Abstract

Keratitis is a condition in which the eye's cornea, the front part of the eye, becomes inflamed. It is caused by Bacteria, Viruses, Fungi and Parasites. This study investigate that the evaluation the microbial diagnosis and Phylogenetic analysis of 16SrDNA and 18SrDNA sequences of bacteria and fungi from infectious eye sample of keratitis patients. In this study 250 consecutive outpatients 143 males and 107 females were with corneal ulcerations were examined in the Eye Clinic of the General Hospital of Tiruchirappalli. Patients were examined with a slit lamp biomicroscope. The Bacteria and fungus were isolated in eye swabs of keratitis patients. The bacterial and fungal genes were sequenced by using 16S rDNA and 18S rDNA- PCR. These sequences were identified using BLASTn (Basic Local Alignment Search Tool nucleotide) and deposited in the GenBank (NCBI) with the accession numbers Staphylococcus aureus (JN378392), Staphylococcus epidermidis (HQ404365), Micrococcus sp.(HM20450), Streptococcus viridians(JN378393), Moraxella sp.(JQ039348), Propionibacterium acnes (JQ039349), Acinetobacter baumanii (JN652129), Citrobacter koseri (JN652127), Pseudomonas aeruginosa(JQ039350), Proteus mirabilis (FJ655896), Klebsiella sp.(JN652128) , Fusarium sp.(JQ412816), Aspergillus sp.(JX204747), Sebipora aquosa (submitted) and Byssochlamys nivea (Submitted). The Sequences were analysed and aligned by Clustal W. It concluded that the result may be useful in characterizing the micro evolutionary mechanisms of the species for researchers.

Keywords: keratitis disease, CLUSTALW, Blastn, Bacteria.