Abstract

LIGAND DECORATED NANOPARTICLES OF COLCHICINE FOR TARGETING BREAST CANCER CELLS

Sandhya, Devender Chauhan, Dr. Dinesh Kaushik*

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Abstract

The field of nanotechnology has rapidly evolved as evidenced by the fact that there are more than 150 ongoing clinical trials investigating the efficacy of nanotechnology based drug delivery carriers targeting cancer. Breast cancer refers to the erratic growth and proliferation of cells that originate in the breast tissue. Anticancer drugs, which have been developed from natural products are Colchicine. Colchicine one such natural alkaloid is colchicine, extracted from the stem of saffron meadow (Colchicine autumnale). Folate conjugated gelatin was synthesized using covalent coupling technique. Physicochemical characterization of nanoparticles are spectra (FTIR), Differential scanning calorimetry (DSC),Transmission electron microscopy (TEM), Powder X-ray diffraction (PXRAD), Particle size, Encapsulation efficiency and drug loading capacity, In vitro drug release, Cytotoxicity assay, Cellular uptake in cancer cells. We have synthesized ligand decorated nanoparticles using folic acid and gelatin for targeting breast cancer cells. In vitro cytotoxicity and cellular uptake study confirmed that CLC-FA-GNs with greater efficacy compared with CLC alone and CLC-GNs may potentially be used for targeting breast cancer cells.

Keywords: Nanoparticles, Gelatin, Colchicine.