Abstract

PHARMACEUTICAL-ANALYTICAL STUDY OF MANIKYA RASA W. S. R. TO ITS TOXICITY STUDY IN RATS.

Dr. Anju Thaware*, Dr. Khushal Dahule and Dr. Vineet Bharne

.

Abstract

Rasashastra is one of these branches of Ayurveda which specifically deals with minerals, metals and herbo-mineral preparations along with their therapeutic uses. Kupipakwa preparations are prepared by unique pattern of heating in a specialized instrument and are very important part of Rasashastra. Manikya rasa is one such Kupipakwa preparation mentioned in Rasatarangini, which contains Hingula, Gandhaka, and Haritala as its main ingredients. Toxicity studies attempt to provide evidence about the safety of the drugs regarding which there is fear about its toxicity. In the standard protocol of clinical research, initially the safety of a drug is evaluated by doing preclinical studies in animals and then only it is used in human beings. So the safety and toxicity profile of the drug is important which is achieved through animal experimentation. Hence for the safety evaluation of the prepared Manikya Rasa, the toxicity study were done on albino rats. This work were carried out in three section 1. Pharmaceutical Study 2. Analytical Study 3. Toxicity Study The pharmaceutical procedures adopted in this study are Shodhana, Bhavana and Kupipakwa procedure. Shodhana is done for Hingula, Gandhaka and Haratala. In addition, it makes the drug suitable for further processes. The Raw drugs were subjected to Shodhana and processed by Palasha Pushpa Swarasa. Manikya Rasa was prepared by Kupipakwa method. Then Manikya Rasa (containing Hingula, Gandhaka and Haratala) was subjected to experimental study to evaluate its safety. Analytical study of Manikya rasa were done and toxicity study was divided into three parts (1) Ponderal Changes (2) Biochemical study (3) Histopathological study. All parameters were conducted in albino rats for 7 days. The toxicity study was done as per the WHO norms on acute level. In acute toxicity study the drug was administered at very higher doses (20mg, 40mg, 60mg) for short duration (7 days). One group as control and three groups for different concentrations of Manikya Rasa were taken for the whole study. Manikya Rasa did not show any significant toxic effects on vital organs of albino rats in Acute Toxicity study when 10 times higher dose than therapeutic dose was given.

Keywords: Manikya Rasa, albino rats, Kupipakwa, Hingula, Gandhaka and Haratala.