Abstract

ESBL, MBL AND AmpC DETECTION IN MULTIDRUG RESISTANT PSEUDOMONAS AERUGINOSA (MDRPA) AND PANDRUG RESISTANT Pseudomonas aeruginosa (PDRPA) ISOLATED IN TERTIARY CARE HOSPITAL

Vijay Mane*, A D Urhekar and Nitin Goel Insan

Abstract

Pseudomonas aeruginosa is a leading cause of nosocomial infection.The rise of antibiotic emergence of resistance may vary with differentantibiotic treatments. A variety of ß-lactamases which include ESBLs,AmpC ß-lactamases and metallo-ß-lactamases, have emerged as themost worrisome mechanism of resistance among the gram negativebacteria, which pose a therapeutic challenge to the health care settings.Materials and Methods: A total of 190 clinical isolates ofPseudomonas aeruginosa were studied. Detection of AmpC betalactamasewas performed by three-dimensional method, whereasdetection of ESBL was done by the combined disk diffusion method asper Clinical and Laboratory Standards Institute (CLSI) guidelines andMBL were detected by the combined disc diffusion test.

Keywords: .