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Abstract

IN VITRO ANTI THIAMINE ACTIVITY OF AMARANTHUS SPINOSUS LEAVES OF SIKKIM HIMALAYA: EFFECT OF SOLVENT, TEMPERATURE, PH AND DURATION OF EXTRACTION PROCESS

Tanaya Ghosh and Prasanta Kumar Mitra*

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Abstract

Effect of solvent, temperature, pH and time on extraction process of anti-thiamine factor present in Amaranthus spinsus leaves was studied. Results showed that extraction of the leaf-extract with methanol at 50º C for 15 minutes at pH 3.0 had maximum anti-thiamine activity in in vitro experiments.

Keywords: Extraction process, Anti-thiamine factor, Amaranthus spinosus.


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