Abstract

ISOLATION OF AN ANTI THIAMINE COMPOUND FROM AMARANTHUS SPINOSUS LEAVES OF SIKKIM HIMALAYA

Tanaya Ghosh and Prasanta Kumar Mitra*

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Abstract

Recently we have shown that Amaranthus spinosus (A. spinosus, Family-Amaranthaceae) leaves of the period May – June had maximum in vitro anti thiamine effect. Aim of the present work was, to isolate anti thiamine compound from the leaves of A. spinosus. From the local market A. spinosus leaves were collected during May – June and identified by the taxonomist. Solvent extraction and acid hydrolysis were done followed by solvent treatment, chromatographic experiments and crystallization. A compound was crystallized. In vitro anti thiamine activity of the isolated compound was checked by standard method. Results revealed that 100 mg of the isolated compound could inactivate 33.5 mg of thiamine hydrochloride in in vitro experiment.

Keywords: Amaranthus spinosus leaves, Isolated compound, Anti thiamine activity.