Abstract

ANTIOXIDANT, ANTI-INFLAMMATORY AND ANTI-DIABETIC POTENTIAL OF VITIS VINIFERA LINN LEAF EXTRACTS

Lateef Ahamd Dar, Suhail Ahmad Mir and Sabeeha Shafi*

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Abstract

Inflammation is considered as an overwhelming burden to the healthcare status and is associated with significant number of diseases such as cancer, chronic lower respiratory disease, inflammatory bowel diseases, diabetes, asthma, osteoporosis, nephritis etc. Diabetes mellitus is accompanied with long-lasting damage, dysfunction, and failure of different organs, especially the kidneys, heart, nerves, eyes, and blood vessels. The aim of this study was to evaluate various leaf extract fractions of Vitis viniferafor antioxidant, anti-inflammatory and antidiabetic potential. Membrane stabilization, proteinase inhibition and albumin denaturation methods were used to assess antiinflammatory activity. The antidiabetic activity was evaluated by nonenzymatic glycosylation of hemoglobin and alpha amylase inhibition methods. The antioxidant activity was evaluated by ferric reducing/antioxidant power (FRAP), 2,2- diphenyl-1-picrylhydrazyl (DPPH), and radical mediated DNA damage methods. The hydroalcoholic extract showed overall significant antioxidant, anti-inflammatory and antidiabetic activity in a concentration dependent manner compared to ethyl acetate and petroleum ether extracts. This might be due to the presence of high phenolic and flavonoid content in the hydroalcoholic extract. The results are suggestive of potential in-vitro antiinflammatory, antidiabetic and protective activity of hydroalcoholic extract of Vitis vinifera leaf.

Keywords: Vitis vinifera, DNA damage, Anti-inflammatory, Antidiabetic, Gel electrophoresis.