Abstract

TITLE: IN VITRO AND IN VIVO ANALYSIS OF ENZYMATIC ANTIOXIDANT POTENTIAL OF RICINUS COMMUNIS LEAVES IN ALLOXAN INDUCED DIABETIC SWISS ALBINO MICE.

Nitika Verma*, Swaati Sharma, Veena Garg

Abstract

The antioxidative bioefficacy of hydro-ethanolic extract of Ricinus communis (RC) leaves was tested by evaluating its enzymatic antioxidant potential both in vitro and in vivo. The in vitro tests included assay for Superoxide Dismutase (SOD), Catalase (CAT), and Glutathione Peroxide (GPx) activity. The in vivo experiments were performed on male Swiss albino mice. The experimental animals were divided in to 4 groups of 7 mice each viz.: Normal Control (NC), Diabetic Control (DC), Leaf extract Treated (LT) and Glibenclamide Treated (GT). The animals of groups DC, LT and GT were made diabetic by a single intra-peritonial injection of Alloxan monohydrate (150mg/kg body weight) and thus were under diabetes caused oxidative stress. In a 45 day long experiment, the group NC and DC were kept untreated and were given only normal saline. Whereas, group LT was treated with Ricinus communis Leaf Extract (RCLE) (300mg/kg body weight) and group GT with a standard drug Glibenclamide for 45 days. The antioxidant assays for CAT, SOD and GPx were carried out on Liver, Kidney and Pancreas homogenates of experimental animals after the 45 day treatment. The in vitro assays demonstrated that the antioxidant ability of the extract increased with its rising concentration. In vivo assays showed significantly reduced enzymatic antioxidants in mice of diabetic group, as compared to the normal group. The treatment with hydro-ethanolic leaf extract helped in significantly normalizing the disturbed enzymatic antioxidants in diabetic mice which was not possible by Glibenclamide treatment.

Keywords: .