Abstract

EVALUATION OF ANTIDIABETIC POTENTIAL OF POLYHERBAL FORMULATION (DIARONIL) IN ALLOXAN INDUCED RAT MODEL

Amrutha Raj P.*, Jayachandran T. P. and Soniraj V. S.

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Abstract

Diabetes Mellitus is a chronic metabolic disorder and a leading cause of morbidity and mortality in the world. In the present study it was investigated for impaired glucose tolerance by oral glucose tolerance test and oral antidiabetic activity of polyherbal formulation in alloxan induced diabetes mellitus rat models using glibenclamide as standard. The acute toxicity studies of polyherbal formulation did not show any toxic symptoms in doses up to 2000mg/kg over 14 days. In oral glucose tolerance test, the polyherbal formulation increased the glucose tolerance in dose dependent manner. Antidiabetic screening against alloxan induced model, the investigational drug was administered for 21 consecutive days and the effect of polyherbal formulation on blood glucose level was studied at regular intervals. At the end of the study the blood samples were collected from all the animals for biochemical estimation, and the animal was sacrificed and the pancreatic tissue was collected for histopathological analysis. Polyherbal formulation showed significant antidiabetic activity at 200 and 400 mg/kg respectively, and the effect was comparable with that of glibenclamide. The antidiabetic activity of polyherbal formulation was further supported by biochemichal analysis.

Keywords: Polyherbal Formulation, Antidiabetic, Oral Glucose Tolerance Test, Alloxan, Glibenclamide.