Abstract

EVALUATION OF ANTI-RHEUMATOID ARTHRITIC ACTIVITY OF LEEA MACROPHYLLA ON EXPERIMENTAL ANIMALS

Dr. D. V. Kishore*, Tabassum Unnisa*, Dr. Mohd. Khasim, Dr. Sridhar Babu, Shabana Khanam

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Abstract

Rheumatoid arthritis (RA) is a chronic, inflammatory disorder and systemic autoimmune sickness which could affect many tissues and organs, however principally attacks flexible (synovial) joints. This system produces an inflammatory reaction of the pill (capsule) around the joints and secondary swelling of the synovial cells. There are different types of arthritis, the rheumatoid arthritis because of the presence of pro-inflammatory markers, cytokines and leuokotrienes. The present study is done to analyse the anti-arthritic activity of crude methanol extract of leaves of L.macrophylla Roxb in rat models. Antiarthritic activity of L. macrophylla extracts (In-ivivo) turpentine oil induce arthritis and formaldehyde induce arthritis in rat model was done at 200 and 400 mg/kg, body weight for 9 days. Paw Edema, was evaluated and corelate with standard and disease control group, Invitro models are: Effect of Sample T on human red blood cell membrane stabilization activity, Effect of Sample T on human red blood cell protein denaturation activity, Effect of Sample T on human red blood cells ProteinASE inhibitory activity is done at 50, 100, 250, 500, 1000, 2000mcg in ml. Conclusion: It can be conclude that methaolic extract of Leea macrophylla possesses significant concentration-dependent anti-arthritic activity. It also brought about the inhibition of inflammation caused by Farmaldehyde and turpentine oil, confirming its anti-arthritic activity.

Keywords: Arthritis, Leea macrophylla, Methanolic extract, Anti-arthritic activity, Wistar albino rats, formaldehyde, and turpentine oil ProteinASE. HRBC.