Abstract

PHYTO-CHEMICAL SCREENING, FT-IR AND GC-MS ANALYSIS OF HYBANTHUS STELLARIOIDES (DOMIN) P. I. FORSTER (VIOLACEAE)

Lingaraj Kulkarni*, Pradeep Bhat and Ganesh Hegde (Rtd)

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Abstract

The purpose of the current study is to monitor the phytochemical constituents in H. stellarioides plant extract. Phytochemical screening of the sequential extracts were used for analysis which showed the presence of bioactive compounds like alkaloids, flavonoids, phenols, tannins, saponins, terpenoids and carbohydrates. Ultra violet visible spectroscopy was used to recognize quantitative determination of different analytes by using wavelength and absorbance values. FT- IR analysis was used to identify the functional groups of the compounds and confirmed the presence of –OH, C-H, C=C (Alkene) and C-O (Ether) in methanol extract. GC-MS analysis for methanolic plant extract was done. The compound 3-methyl-4-(phenylthio)-2-prop-2- enyl-2,5- dihydroxy; 3-trifluoroacetoxydodecane; cyclopenta (c) furo (3’-2’-h) (1) benzop; 3- hexadacycloxylocarbonyl-5-(2-hydroxy); 1,3-butanediol, diacetate; cholest-7-en-3 beta, 5 alpha- diol-6 alpha benz and 2-cyclopentene-1-one, 5-hydroxy-2,3-demethyl-. are possessed analgesic, anti-inflammatory and antipyretic activities, antimicrobial and antioxidant activities. Natural Chemical compounds of the medicinal plants have provided numerous clinically useful drugs in the treatment of chronic or acute ailments and still remain as an essential component in the search for new medicines.

Keywords: Hybanthus stellarioides, phytochemical screening, UV-Vis-spectroscopy, FT-IR, GC-MS analysis.