Abstract

ANGIOTENSIN I-CONVERTING ENZYME GENE POLYMORPHISM IN PATIENTS WITH CHRONIC RENAL FAILURE

Mohemid M Al-Jebouri* and Huda R Al-Alwani

Abstract

Background: The Renin-Angiotensin system (RAS) is a key regulator of both blood pressure and kidney functions and their interaction. In such situation, genetic variability in the genes of different components of RAS is likely to contribute for its heterogeneous association in the renal disease patients. Angiotensin converting enzyme-1 (ACE-1) is an important component of RAS which determines the vasoactive peptide Angiotensin-II. Methods: In the present study, we have investigated 100 ESRD patients and 50 normal healthy controls to deduce the association between ACE gene polymorphism and ESRD. A total of 50 normal healthy controls were also genotyped for ACE I/D polymorphism. The criterion of defining control sample as normal was totally based on the absence of any kidney disease determined from the serum creatinine level. Genotyping of ACE I/D was assayed by polymerase chain reaction (PCR) based DNA amplification using specific flanking primers. Results: of 100 patients with chronic renal failure with different causes, the DD genotype was 56%, II genotype 13%, ID was 28% and negative results was 3%. The control group results was DD 26%, II 26%, ID 22%, and negative results was 26%. Conclusion: the results in this study of patients with chronic renal disease indicate that presence of the D allele in the ACE genotype may be of particular importance as a predictor of high rate of progression to ESRD.

Keywords: chronic renal failure, ACE, polymorphism.