Abstract

SERUM LEVEL OF IL-6, IL-1? AND TNF-? IN PATIENTS OF CHRONIC RENAL FAILURE WITH MAINTENANCE HAEMODIALYSIS

Mohemid M AL-Jebouri and Huda R Al-Alwani

Abstract

Background: Chronic inflammation is a common feature of end stage renal disease which carries a heightened risk of atherosclerosis and other co-morbid conditions. Dialysis treatment per se can bring additional risk factors for inflammation such as increased risk of local graft and fistula infections, impure dialysate or bio-incompatible membranes. The present study was designed to determine the level of proinflammatory cytokines in chronic renal failure and whether a hemodialysis session leads to an acute substantial alteration in the plasma levels of the proinflammatory interleukins IL-6, IL-1β and tumor necrosis factor TNF-α. Methods: To determine the plasma level of IL-6, TNF-α, and IL-1β, 100 patients with ESRD were studied. The patients include 42% females and 58% males. Patients aged group between 18 to 80 years (mean=50.35±16.51). The control group aged (45.48±15.05). The blood samples (100 patients) were collected prior haemodialysis session and 50 control persons. The level of these cytokines in seum using ELISA method was estimated. Results: the level of these cytokines in serum of patients recently had dialysis treatment were high while the level of IL- 6 was decreased progressively with dialytic age. The level of TNF-α and IL-1β also decreased with dialytic age but not as in IL-6. Conclusion: the results presented here indicated that prolonged treatment with dialysis can be considered a form of chronic stress that causes the progressive activation of monocytes which ultimately leads to monocyte exhaustion and dysfunction.

Keywords: IL-6, IL-1?, TNF-?, chronic renal failure, haemodialysis.