Abstract

ISOLATION OF AN ANTIOXIDANT COMPOUND FROM CLERODENDRON INFORTUNATUM LEAVES

Tanaya Ghosh*, Prasenjit Mitra and Prasanta Kumar Mitra

.

Abstract

Clerodendron infortunatum (C. infortunatum) has several pharmacological properties including antioxidant activity. Aim of the present work was to isolate antioxidant compound from C. infortunatum leaves. Samples of C. infortunatum leaves were collected from the local market and identified by the taxonomist. Methanol extract of the leaves was prepared and processed for isolation of antioxidant compound. Acid hydrolysis, solvent treatment, chromatographic experiments followed by crystallization were done to isolate a compound. In vitro antioxidant activity of the isolated compound was measured by superoxide anion generation with the help of xanthine-xanthine oxidase assay, linoleic acid peroxidation assay as well as by DPPH photometric assay. Isolated compound showed significant in vitro antioxidant activity which was comparable to that of quercetin, a synthetic antioxidant. The isolated compound, therefore, may be used as natural antioxidant.

Keywords: Clerodendron infortunatum leaves, Isolation of antioxidant compound, In vitro antioxidant activity of the compound.