Abstract

IMMUNOLOGICAL STUDY OF GASTRIC-ULCER PATIENTS INFECTED WITH HELICOBACTER PYLORI

Suha Talib Yahya Al-Jumaily, *Rajwa Hasen Essa, and Mohammed Issa Muhsin

Abstract

This study includes collection of biopsy and blood specimens from patients with age ranged between 17-76 years that suffering from dyspepsia, gastritis, duodenal ulcer, and gastric cancer during the period between December 2011 to April 2012 at AL-Kadhymia Teaching Hospital. This study also involved twenty healthy persons as a control group. Patients group were subjected to gastroduodenal endoscopy, gastric biopsy were taken from them for bacteriological investigations. Blood samples were also collected for immunological tests, while only blood samples were collected from control group. The Detection of Helicobacter pylori was performed using several laboratory investigations such as biopsy urease test, bacterial culture, precipitation test, and serological test which include immunoglobulins and complement quantitation and enzyme Linked Immunosorbent Assay (ELISA) for Interferon gamma (IFN- γ) and Interleukin-8 (IL-8) detection. Outer membrane proteins were extracted from these bacteria using Murphy method and the concentration of proteins were calculated by using spectrophotometer, then proteins detected and the molecular weight of proteins were evaluated by using electrophoresis. The stydy showed the prevalence of H. pylori in (44.56%) of 92 patients. There was no significant relation between age factor and distribution of H. pylori, but there is a significant relation between gender factor and distribution of H. pylori. The result of biopsy culture was (7.31%) of (41) infected patients, while biopsy urease test and Rapid anti H. pylori test gave (44.56% and 64.13%) respectively of 92 patients. Electrophoresis results revealed ten bands which ranged between (16.914 – 340.476) KDa with protein concentration of (2.76028) mg/ml. Outer membrane proteins were extracted from H. pylori isolates and used as antigen to detect specific H. pylori antibodies using precipitation test in which the formation of precipitating line considered as a positive result. High titer of IgG, IgA and C3 appeared in patients group, while a normal titers of IgM and C4, as a result of immunoglobulins and complement quantitation. Enzyme-Linked Immunosorbent Assay for detection of IFN-γ and IL-8 revealed statistically significant high level of both in compared with control group.

Keywords: Helicobacter pylori, Gastric, immunological aspects.