Abstract

CALLUS INDUCTION AND ORGANOGENESIS IN CAPSICUM ANNUUM L. CV PUSAJWALA AND G4

Srinath Rao*# and Prathiba Sangapure

Abstract

Callus induction and plant regeneration in chilli pepper (Capsicum annuum L.) cv pusajwala and G4 was investigated. Cotyledon and hypocotyl explants excised from 10-12 days old seedlings were cultured on Murashige & Skoog’s (MS) medium supplemented with different concentrations of growth regulators like 2, 4-D, NAA, BAP, and Zeatin. Both the explants were found suitable for induction of callus on MS medium supplemented with NAA at 2.0mg/l. Hypocotyl was found to be a better explant for the induction of maximum callus. Healthy multiple shoots were obtained from the cut ends of cotyledonary leaf segments for both the varieties on MS containing 1.0 mg/l BAP and 0.1 mg/l IAA. The maximum root induction from the regenerated shoots was achieved on half the strength MS medium supplemented with 0.2 mg/l IAA. The in vitro grown plantlets were successfully transplanted into soil where they flowered and produced fruits identical to those developed by control plants.

Keywords: Chilli, callus, multiple shoots, 2, 4-D, BAP