Abstract

DEVELOPMENT AND VALIDATION OF A NOVEL AND SIMPLE RPHPLC METHOD FOR ESTIMATION OF TANGERETIN IN ORANGE PEEL POWDER EXTRACTS

Sachin Bhusari*, Amit Rindhe and Pravin Wakte

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Abstract

A rapid and sensitive reversed phase high-performance liquid chromatography (RP-HPLC) method was developed for the estimation of tangeretin. The method was validated according the Q2 (R1) guidelines of International Conference on Harmonization (ICH) with respect to system suitability, linearity, range, LOD, LOQ, accuracy, precision. The chromatographic analysis was performed on Shimadzu HPLC instrument using a C18, 250mmx4.6mm, 5 μm dimensions Column and mobile phase comprising Methanol and water were mixed in the ratio of 60:40 v/v at the flow rate of 1 ml/min. The column eluent was monitored at 327 nm. The total run time was 10 min and the average retention time of b tangeretin was found to be 7.498 min. The method showed excellent linear response with correlation coefficient values (R2) of 0.999 which were within the limit of correlation coefficient (R2 >0.999). The intra- and inter-day accuracy and precision values for all the analytes were within the acceptable range. The LOD and LOQ were 0.1325 and 0.9945 ng/ml. The developed method was found to be robust. The method was successfully used for the quantitative analysis of herbal plant containing Orange peel extract. Due to sensitivity, proposed method can be used industrially for routine analysis of Orange peel samples.

Keywords: Tangeretin, HPLC method development, RP- HPLC.