Abstract

IN VIVO ANTI ARTHRITIC ACTIVITY OF BRASSICA NIGER

Priyanka Dangi*, Rahim Khan, Dr. Brajesh Ku. Arjariya, Rahul Dubey and Dr. Mohd Jalaluddin

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Abstract

The term arthritis is used to refer to several different musculo-skeletal problems. These include osteoarthritis, rheumatoid arthritis, juvenile arthritis, systemic lupus, gout, and fibrositis. The plant of Brassica niger have long been used traditionally to treat joint pain. Though, it has not been pharmacologically assessed for rheumatoid arthritis. The current study explores anti-arthritic activity and phytochemical analysis of different solvent extract and fractions. In the present study, extraction was performed using continuous hot percolation ‘Soxhlation’. Dried pulverised leaves of Brassica niger were placed in thimble of Soxhlet apparatus. Soxhlation was performed at 60°C using Petroleum ether (40 - 60ºC) as nonpolar solvent at first. Freud's adjuvant induced arthritis model was used to assess the anti-arthritic activity in albino rats. Volumes of injected hind paws of rats were measured before and at 7 days interval after adjuvant inoculation by using water displacement plethysmograph. The present preclinical study has revealed evidences for the traditional use of Brassica niger in the treatment of arthritis The study concludes that Brassica niger exhibited significant anti-arthritic activity at the dose 200 mg/kg than the lower dose 100 mg/kg. Taken together, these results support traditional use of Brassica niger as potent anti-arthritic agent that may be proposed for arthritis treatment.

Keywords: arthritis, anti-inflammatory, anti-arthritic, Brassica niger.